Skin Irritation (MTT ET-50)

的 in vitro 表皮 MTT ET-50 assay is used as a screen to assign expected 在活的有机体内 irritancy responses based on the time-to-toxicity (ET-50) results obtained with 表皮. This assay is also used to rank order test formulations according to their skin irritation potential.

During product development of new personal care or cosmetic formulations, the skin irritation potential is evaluated to identify substances which may induce adverse skin reactions. Understanding the potential of topically applied chemicals or formulations to induce skin irritation is an important consideration to avoid exposing human volunteers to potentially irritating materials during clinical testing.

365体育网投 offers the 表皮 Skin Irritation Test as a non-GLP service.

Protocol

Test Model	表皮
Replicates	N=2 tissues per test condition
Exposure Time	2, 5 and 18-hour topical exposure to 100µl or 100mg of test material per tissue
Assay Controls	Negative Control – diH₂O Positive Control – 1% Triton
Endpoints	MTT Tissue Viability Assay
Data Delivery	ET-50 values Expected 在活的有机体内 irritancy category (i.e. strong, moderate, mild, non-irritating)